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IIS 7.5 app pool domain account issue

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I've been having problems with a dev server and I hope someone could shed some light on this.

MVC 4 .Net 4.5 app, challenge/response only, no impersonation, on an IIS 7.5 box using an AD domain service account as the app pool account targeted at that application only.

I'm trying to connect to SQL server on a separate box using the app pool account (domain\account). The account is registered in security for SQL and the relevant databases and applied to various stored procedures within those databases. Basically a standard set up that I've done a good few times before.

It just won't connect, as it keeps trying to use the machine$ account which I don't want to use. It won't even connect if I put a user id and password in the connection string and I'm now stumped. Has anyone got any pointers to solve this? Help much appreciated.

 

Edit to add: if I add use my account as the app pool identity account I get straight into the database, but of course that's not much use.

Oh.... windows identity is the app pool account (domain\account), user identity the logged requesting user


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