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HTTP Error 404.17 - Not Found [Yes the same issue again]

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I have a web service developed in .Net 4.0 in VS 2010. The tested web service is deployed on our DEV Win 2008 Server (having .net 2.0 and 4.0) in IIS 7.5 in its own AppPool. Post installation, I changed the AppPool setting for the WebService to use .Net version 4.0, Enable 32-bit is set to true and Managed Pipeline is set to Classic. When I try accessing the webservice xxx.asmx page in browser (I have enabled the directory browsing) I get "HTTP Error 404.17 - Not Found" error. Steps tried: 1. I saw a couple of links suggesting registering of aspnet_iisreg. I used suggestion from http://stackoverflow.com/questions/1078197/determining-if-asp-net-is-properly-registered and got the following C:\Windows\Microsoft.NET\Framework64\v4.0.30319>aspnet_regiis -lv 2.0.50727.0 C:\Windows\Microsoft.NET\Framework64\v2.0.50727\aspnet_isapi.dll 4.0.30319.0 c:\Windows\Microsoft.NET\Framework64\v4.0.30319\aspnet_isapi.dll 4.0.30319.0 c:\Windows\Microsoft.NET\Framework\v4.0.30319\aspnet_isapi.dll 2. Some links mentioned enabling 32 bit in the app pool, that is already done. 3. Some links were suggesting visiting the Handler Mappings. When I do that I do not see any mention of .net 4.0 isapi dll. 4. The ISAPI and CGI Restrictions does show "C:\Windows\Microsoft.NET\Framework64\v4.0.30319\aspnet_isapi.dll" with Restriction as Allowed. @Help What configuration could be missed out? Do let me know if I can furnish any additional details to narrow down options for solution. This is my first post on the forum. Pardon the formatting...

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